Selection of oleaginous yeasts isolated from sugarcane bagasse as the potential producers of single cell oils and other metabolites

Abstract Purpose The microorganisms that possess diverse and improved traits for biotechnological applications provide an opportunity to address some of the current industrial challenges such as sustainability fuel energy food. aim this study was isolate select potential yeast isolates from sugarcane bagasse single oil production. Methods oleaginocity yeasts confirmed through a gravimetric analysis lipids, Nile red, sulfo-phosphovanillin (SPV) gas chromatography method fatty acid methyl esters (FAME). identification selected carried out 5.8S ribosomal internal transcribed spacer (ITS) 26S DNA (rDNA) sequences. carbohydrate protein contents were estimated using phenol-sulfuric hot trichloroacetic method, respectively. Results accumulated lipids between 28% 67% dry cell weight, 22–33% based on SPV assay, qualifying them oleaginous yeasts. identified Candida ethanolica Pichia manshuriica . contained high levels acids in following order: oleic acid, palmitic stearic linoleic which made up 82% total lipids. Conclusion All six strains have produce oils other metabolites. composition species found be suitable biofuel, nutraceutical, food industry. require evaluation lignocellulosic hydrolysate production cost effective.